How manure can be transformed into animal feed

In her doctoral project, Clarisse Uwineza demonstrated how food waste and animal manure can be converted into valuable volatile fatty acids. These acids can then be used as a substrate to cultivate protein-rich fungal biomass, which in turn can be used to produce animal feed.

Early in her studies, she developed an interest in creating sustainable methods for managing waste and by-products. Her research allowed her to combine her interests in both biotechnology and resource recovery.

Organic waste, such as food waste and manure from agriculture, is a growing environmental issue. However, by using a method known as anaerobic digestion, where microorganisms break down waste in an oxygen-free environment, it is possible to extract these volatile fatty acids. These acids, along with nutrients such as nitrogen and minerals, can then be used to cultivate a specific fungus: Aspergillus oryzae. The fungus grows into a biomass rich in protein, minerals, and dietary fibre, and is also easily digestible for animals.

“My work shows that organic residues can serve as a valuable raw material for sustainable protein production, helping to close the loop in a circular economy and potentially reducing the use of protein sources such as soy and fishmeal, which currently form the basis of animal feed production,” said Clarisse Uwineza.

Growing surprisingly effective

“One thing that surprised me was how effectively the fungal biomass grew on waste-derived acids, even though some of these acids are toxic at high concentrations”, she continued.

By developing a controlled method for adding the volatile fatty acids, she was able to ensure the fungus grew gradually and safely, resulting in higher yields and improved quality.

In addition to animal feed, the fungal biomass can also be used in the production of bio-based materials, such as biodegradable plastics or as an alternative to leather. The results show that emissions can be reduced, resources can be better utilised, and new opportunities can be created within the circular bioeconomy.

The project directly supports several of the UN’s Sustainable Development Goals, including Goal 12 on responsible consumption and production, Goal 13 on climate action, and Goal 2 on ending hunger.

Further reading

Clarisse Uwineza earned her PhD in Resource Recovery at the University of Borås.

Read the doctoral thesis: Cultivation of Edible Filamentous Fungi Utilising Bio-based Volatile Fatty Acids

Main supervisor: Professor Mohammad Taherzadeh

Co-supervisor: Associate Professor Amir Mahboubi Soufiani

Opponent: Professor Yifeng Zhang, Department of Environmental and Resource Engineering, Technical University of Denmark

Learn more about the research field Resource Recovery

Learn more about the Biotechnology research